: Once inside the cell, natural enzymes called esterases chop off the AM group. This "traps" the dye inside the cell and makes it sensitive to calcium.
Fura-2AM is a that allows scientists to see and measure changes in intracellular calcium concentrations ( Te-AИ™ Fura
: Many researchers add this detergent to help the dye dissolve better in the cell-loading liquid. : Once inside the cell, natural enzymes called
: Observing how new medications affect cell signaling and heart muscle contractions. : Observing how new medications affect cell signaling
: The "AM" stands for acetoxymethyl ester . This chemical modification makes the dye membrane-permeant, allowing it to easily slip through the fatty outer layer of a cell.
). It is widely used in labs because it is "ratiometric," meaning it provides highly accurate readings by comparing light emitted at two different wavelengths. How it Works
Information on like Fluo-4 for faster imaging.