The story follows Peter Parker, a socially awkward high school student who gains superhuman abilities after being bitten by a genetically engineered "super-spider." Following the tragic death of his Uncle Ben—which Peter could have prevented—he realizes that with great power comes great responsibility and begins fighting crime as Spider-Man. Act I: The Rise of a Hero

Peter begins his journey by testing his new powers in an underground wrestling circuit to win money for a car. After his uncle is killed by a carjacker, Peter tracks the criminal to a warehouse, only to discover it was the same man he let escape earlier. Driven by guilt, he dedicates himself to protecting New York City.

In the final showdown at an abandoned cathedral, Peter and Norman engage in a savage fistfight. Norman attempts to kill Peter with his remote-controlled glider, but Peter’s spider-sense allows him to dodge. The glider impales Norman instead, ending the Green Goblin's reign of terror. Peter chooses to remain a lonely hero to keep Mary Jane safe, fully embracing his destiny.

The Green Goblin discovers Spider-Man’s secret identity and attacks those closest to him. He crashes a festival at Times Square, leading to a brutal aerial battle. The conflict peaks when the Goblin kidnaps Mary Jane and holds her hostage atop the Queensboro Bridge.

The Xbox game expands on this period by introducing , who is robbing a bank during Peter's early days. Spider-Man pursues Shocker through the city's sewer system and subway tunnels, eventually defeating him in a climactic battle at a transit station. Act II: Oscorp’s Dark Secret

While Peter balances college life and his crush on Mary Jane Watson, a corporate war erupts. Norman Osborn, CEO of Oscorp, undergoes an experimental super-soldier treatment to save his company’s military contracts. The process drives him insane, creating the .

If you beat the game on Hero or Superhero difficulty, you unlock the ability to play through the entire story as Harry Osborn in the Green Goblin suit, featuring a completely different "alternate reality" script where Harry tries to uncover the truth about his father's death.