Electrophoresis Apr 2026

Used for proteins. The detergent SDS unfolds the proteins and gives them a uniform negative charge, ensuring they are separated strictly by length rather than shape.

It is used to detect abnormal proteins in blood or urine, which can indicate conditions like multiple myeloma or hemoglobin disorders (e.g., sickle cell anemia). Electrophoresis

The process relies on the principle that many biological molecules possess an electrical charge. When placed in an electric field, these molecules migrate toward the electrode with the opposite charge. Used for proteins

Molecules are loaded into a porous gel, typically made of agarose (for large DNA fragments) or polyacrylamide (for smaller DNA or proteins). This gel acts as a molecular sieve. The process relies on the principle that many

Uses narrow tubes instead of gels, allowing for high-speed separation and automation, commonly used in modern DNA sequencing. Real-World Applications