Download Star Bin ✦ Free Forever
For visualizing multiple STAR reports at once, many researchers use MultiQC , which can aggregate these .final.out files into a single, interactive HTML report. alexdobin/STAR: RNA-seq aligner - GitHub
STAR --runThreadN [threads] \ --runMode genomeGenerate \ --genomeDir /path/to/index/ \ --genomeFastaFiles /path/to/genome.fasta \ --sjdbGTFfile /path/to/annotation.gtf Use code with caution. 3. Run Alignment and Generate Report Download STAR bin
STAR --runThreadN [threads] \ --genomeDir /path/to/index/ \ --readFilesIn read1.fastq read2.fastq \ --outFileNamePrefix SampleName_ Use code with caution. 4. Interpretation of the Report For visualizing multiple STAR reports at once, many
Helpful for diagnosing contamination or poor library quality. many researchers use MultiQC
Before you can align reads and generate a report, you must create a genome index from your reference FASTA and GTF files.