en.indiriste.comC18zip ToolsC18zip OptimizationC18zip
WinZip System Utilities Suite icon

C18zip

4.0.0.3.4

3 (1 Vote)
Size :   45.5 MB
Language :   English
License :   Demo
Virus Scan   :   1 / 93
Producer   :  
System   :   Windows (All)
Update     :  
Editor     :  

Peptides dissolved in 0.1% Trifluoroacetic acid (TFA). Wetting Solution: 100% Acetonitrile (ACN). Equilibration/Wash Solution: 0.1% TFA in water. C18zip

💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry. Peptides dissolved in 0

Repeat this cycle to maximize binding to the C18 media. Note: Slow, consistent pipetting is key for high recovery. 5. Washing (Desalting) Aspirate the Wash Solution (0.1% TFA). Dispense to waste. Repeat 3 times to remove salts, buffers, and detergents. 6. Elution Aspirate the Elution Solution (e.g., 60% ACN/0.1% TFA). C18zip

The sample is now ready for (MALDI-TOF or LC-MS/MS).

C18zip

Peptides dissolved in 0.1% Trifluoroacetic acid (TFA). Wetting Solution: 100% Acetonitrile (ACN). Equilibration/Wash Solution: 0.1% TFA in water.

💡 If your sample contains high concentrations of salts or urea, increase the number of wash steps in Section 5 to avoid interference during mass spectrometry.

Repeat this cycle to maximize binding to the C18 media. Note: Slow, consistent pipetting is key for high recovery. 5. Washing (Desalting) Aspirate the Wash Solution (0.1% TFA). Dispense to waste. Repeat 3 times to remove salts, buffers, and detergents. 6. Elution Aspirate the Elution Solution (e.g., 60% ACN/0.1% TFA).

The sample is now ready for (MALDI-TOF or LC-MS/MS).